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KMID : 0613820090190081062
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Journal of Life Science
2009 Volume.19 No. 8 p.1062 ~ p.1066
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Protrusion of N-acetylglucosamine Kinase Clusters into the Base of Excitatory Synapses
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Moon Il-Soo
Cho Sun-Jung
Lee Hyun-Sook
Seog Dae-Hyun
Randall Walikonis
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Abstract
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N-Acetylglucosamine kinase (GlcNAc kinase or NAGK; EC 2.7.1.59) catalyzes the phosphorylation of GlcNAc to GlcNAc-6-phosphate (GlcNAc-6-P). Despite detailed characterization of the enzyme itself, there have been few studies on the expression of NAGK in mammalian tissues. In the rat hippocampal neuron in culture, NAGK-immunoreactivity (IR) formed clusters in somatodendritic domains. In this study we characterized the NAGK clusters that protrude out the long axis of dendritic shafts. By double-labeling of the neurons with antibodies against NAGK and various synaptic proteins, we show that NAGK is positioned at the base of spines, while there were no NAGK protrusions into inhibitory postsynaptic sites. Immunoblot analysis showed that NAGK was included in synaptosomes but not in PSD fractions. Our results indicate that the NAGK clusters at the dendritic periphery protrude into spines.
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KEYWORD
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Dendrite, hippocampal culture, immunocytochemistry, microtubule, NAGK, spine
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